Short communication larvicidal activity of crude extracts. However, research on the larvicidal activity of larrea species against mosquitoes is scarce. The purpose of the present investigation was to explore the larvicidal and repellent activity of ethyl acetate and methanol extracts from leaf, seed and stem of nine plant. Larvicidal bioassay the larvicidal activity of crude extracts of the selected plants was assessed by the protocol of who15 with some modifications and as per the method of rahuman et a16. Larvicidal activity of plant extracts on aedes aegypti l article pdf available in asian pacific journal of tropical biomedicine 23. Evaluation of some moroccan medicinal plant extracts for. Mosquito larvicidal activity of mangrove plant extracts. The results demonstrate mosquitocidal activity of phytochemicals from aloe against ae. Mosquito larvicidal activity of hyptis suaveolens filarial. For the bioassay in a container 25 fourth instar larvae were kept in 249 ml of distilled water with 1 ml of extracts 400 ppm in dmso.
Evaluation of larvicidal efficacy of indigenous plant. The mortality data were subjected to probit analysis to determine the lethal concentrations lc 50 and lc 90. So, the larvicidal and ovicidal activities of crude benzene, hexane, ethyl acetate, chloroform. Larvicidal activity of some plant extracts against mosquitoes. Among the extracts tested, nine exhibited high larvicidal activity with lc 50 24 h ranging from 28 to 325 ppm. A screening for larvicidal activity of plant extracts with some known medicinal attributes could lead to the discovery of new agents for pest and vector control. Therefore, in the present study the larvicidal activity of crude petroleum ether, chloroform and acetone. The plant extracts were screened for larvicidal activity individually and in combination against the larvae of aedes aegypti and culex quinquefasciatus as per who protocol.
The larvicidal activity of the different selected plant extracts were found to be dose depended. Pdf larvicidal activity of medicinal plant extracts against. Ficus benghalensis, larvicidal activity, culex quin quefasciatus, aedes aegypti, anopheles stephensi. This study provides the first report on the larvicidal activity of crude solvent extracts of different mosquitoes.
The extracts of these plant have a few important chemical constituents like carbohydrates, glycoside, and tannins. Issn larvicidal activity of monstera adansonii plant. Larvicidal activity of ricinus communis extract against. The activity of crude plant extracts is often attributed to the complex mixture of active compounds. Plant extracts as potential mosquito larvicides pdf. The present study assessed the role of larvicidal activities of hexane, chloroform, ethyl acetate. Assessment of larvicidal activity of hyptis suaveolens and. Larvicidal activity of silver nanoparticles synthesized. In the present study, the plant extracts exhibited the presence of larvicidal activity table 1 which was a dose dependent effect. Madhura mukadam department of zoology, gogate jogalekar college, ratnagiri, maharastra, india abstractmosquitoes are the major vector for the transmission of malaria, dengue fever, yellow fever, filariasis, schistosomiasis and japanese encephalitis. Studies on larvicidal properties of plant extracts against the fourth instar larvae revealed that extracts of 14 species showed evidence of larvicidal activity. Therefore, it becomes urgent to develop novel alternative tools for vector control. Larvicidal and ovicidal activity of terminalia chebula. Pdf larvicidal activities of six plants extracts against two mosquito.
Larvicidal and repellent activity of medicinal plant. Larvicidal activity of methanolic leaf extracts of plant, chromolaena odorata l. Larvicidal activity of some labiatae lamiaceae plant. Stock solutions of the extracts or oils were prepared in ethanol, and tween80 10 ppm was used as an. Larvicidal activity of some plant extracts against mosquitoes dr. Mosquitoes larvicidal activity of leaf extract of neem azadirachta indica amaragathavalli. Ethanol extracts of the aerial parts from five labiatae lamiaceae species, obtained from antalya, turkey, were tested for larvicidal activity against the house mosquito culex pipiens l. The resistance to chemical insecticides among mosquito species has been considered as a setback in vector control. Pdf larvicidal activity of crude chloroform, dichloromethane and methanol extracts of the leaves and roots of six indian plants, aegle marmelos l.
The soxhlet extraction method had the highest larvicidal effect against ae. Table i asteraceae plant species collected for testing against 4th instar larvae of aedes ochlerotatus fluviatilis lutz, 1904 entry plants common name habitat a bhcbb 1. We carried out larvicidal bioassays with methanolic extract of leaves of chromolaena odorata. Laboratory of chemical ecology, department of fundamental chemistry, federal university of pernambuco ufpe. Mosquito larvicidal activity of mangrove plant extracts and synergistic activity of rhizophora apiculata with pyrethrum against culex quinquefasciatus. The study of mortality is one of the methods of potential larvicidal activity of plants popularly used for this purpose. Larvicidal efficacy of the crude leaf extracts of ficus benghalensis with three different solvents like methanol, benzene and acetone was tested against the early second, third, fourth instar larvae of culex quinquefasciatus, aedes aegypti and anopheles stephensi. Plants may be alternative sources of mosquito control agents. Larvicidal activity of plant and algae extracts, essential oils and isolated chemical constituents against aedes aegypti volume. Larvicidal activity of medicinal plant extracts against anopheles. Larvicidal activity of extracts from three plumbago spp. Larvicidal activity of methanolic leaf extracts of.
Pdf plant extracts as potential mosquito larvicides. Godinho, ashwani kumar abstract mosquitoes transmit malaria, filariasis, dengue, chikungunya, etc. Plant based formulations would be more feasible environmental products with proven potential as insecticide. Issn 23207078 larvicidal activity of lantana camara. The larvicidal properties of 16 extracts of four moroccan medicinal plants. The larvicidal activity of azolla pinnata plant extracts evidently affected the early 4 th instar larvae of aedes aegypti mosquito vectors. Materials and methods the present study has been carried out to assess the larvicidal activity of aqueous extracts of. Third and fourth instar mortality from six concentrations 5, 10, 25, 50, 100 and 200 ppm of each plant. Antifeedant and larvicidal effects of plant extracts. Evaluation of larvicidal activity of medicinal plant. The present study assessed the role of larvicidal activities of hexane, chloroform. In the backdrop of recent revival of interest in developing plantbased insecticides, the present study was carried out to evaluate the larvicidal properties in three medicinal plants growing abundantly in the region of chitheri hills.
Table 1 shows the percentage mortality of the 4th instar larvae of mosquito exposed for 24hrs to various concentrations of acetone extracts of the two plants. Then to each beaker, eight mosquito larvae aedes aegypti were added. The larvicidal activity of the organic extracts was found to be speciesdependent. Larvicidal efficacy of medicinal plant extracts against the vector. Effect of monstera adansonii on 4th instar larvae of culex quinquefasciatus. The mortality data were subjected to probit analysis to determine the lethal concentrations lc 50 and lc 90 to kill 50 and 90 per cent of the treated larvae of the respective. Insectborne diseases remain to this day a major source of illness and death worldwide. This component has potential larvicidal activity due to individual or synergistic effects on larvae. Mosquito is a vector of different diseases like malaria, filariasis and dengue fever.
Larvicidal activity of medicinal plant extracts against aedes aegypti. The present study has been carried out to assess the larvicidal activity of aqueous extracts of ten plants belonging to varied taxonomic groups. In the preliminary screening, potential larvicidal activity of seven plants as crude extracts in different solvents was noted. Evaluation of larvicidal activity of medicinal plant extracts against. This needs a further deep investigation to identify the effective constituents for controlling mosquitoes. Extracts of annona species are potential antimosquito. Larvicidal activities of three medicinal plant extracts were studied in the range of 4. Larvicidal efficacy of medicinal plant extracts against. All plant extracts showed significant larvicidal activity against a.
Pdf larvicidal activity of selected plant extracts and. Pdf larvicidal synergistic activity of plant extracts. Meanwhile, the maceration extraction compounds were recorded with the lc50. Activity of plant extractsessential oils against three. The plant neem azadirachta indica was selected for study.
Chemical composition and larvicidal activities of azolla. Larvicidal activity of selected aloe species against aedes. All these plant extracts were made free from solvent by steam distillation, concentrated by evaporation and were used for the larvicidal testing by standard procedure5. From the stock solution, concentrations activity are methanolic stem extracts of satureja hortensis. It has been increasing development of resistance to synthetic chemicals, so plant extracts may be the alternate source of mosquito control agent. The larvicidal activities lc 50 observed in the two studies are markedly different only sharing values of a.
Culicidae youngcheol yang, i1kwon park, eunhee kim 2, hoiseon lee 3 and youngjoon ahn 2 department of advanced organic materials engineering, chonbuk national university, chonju 561756. Larvicidal activity of medicinal plant extracts against. Larvicidal activity of plant extracts used alone and in. Larvicidal bioassay the larvicidal activity of the extracts or oils was evaluated on the early fourth instar of c. Ochlerotatus togoi, and culex pipiens palens diptera. To test this, larvae were exposed for 24 h to the aqueous plant extract at 1500, 3000, 4500.
You will be redirected to the full text document in the repository in a few seconds, if not click here. Zhu 2008 stated that activities have been focused on plant extracts or phytochemicals as sources of agents to control mosquito. Mosquito control programs, botanical origin may have the potential to eliminate eggs and larvae. Larvicidal activity of plant and algae extracts, essential. Larvicidal efficacy of plant extracts against the malarial. Materials and methods plant extracts vernonia cinerea leaves collected in and around kancheepuram, tamilnadu, india were brought to the laboratory, shade dried under room temperature and powdered using an electric blender. Tinospora rumphii leaf extract is the most effective mosquito larvicide which is manifested by the highest percentage mortality on the larvae of 90% and 93% after 24 and 48 hours respectively.
Pdf larvicidal activity of plant extracts on aedes aegypti l. Evaluations of larvicidal activity of medicinal plant. Plant extracts are safer for nontarget organisms including man. In that context, our study aimed at evaluating the insecticidal activity against ae. The results of larvicidal activity clearly showed that the hexane extract of l. Green synthesis of silver nanoparticles using solanum. Plasmodium spp, the vector mosquito has to be controlled. All plant extracts showed moderate toxic effect on anopheles. Mosquito repellent and larvicidal activities of acalypha. In order to develop an environmentfriendly botanical mosquito larvicide alternative to the chemical larvicides, extracts were made from the leaves of hyptis suaveolens, lantana camara, nerium oleander, and tecoma stans with three organic solvents. Larvicidal activity of methanolic leaf extracts of plant, chromolaena.
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